Cell proliferation and activity detection

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2022-06-08 17:58:53

One. Experiment Principle

Cell proliferation is the division process of cells under the action of cycle regulators through a series of complex reactions such as DNA replication, RNA transcription and protein synthesis. It is the basis of biological growth, development, reproduction and inheritance. Cell proliferation detection is widely used in molecular biology, immunology, tumor biology, pharmacology and other research fields. It is an important method to evaluate cell metabolism, physiology and pathology.

Two. Application Introduction

At present, the method of BrdU labeling newly synthesized DNA has been widely used in the fields of tumor biology, genetics, molecular biology and so on.

Compared with traditional immunofluorescence staining, EdU reaction is very fast and can be completed in a few minutes without DNA denaturation. It only needs a few simple steps, making tissue imaging easier. Therefore, it is also suitable for high-throughput screening tests, especially for screening experiments of siRNA, miRNA, small molecular compounds and other drugs.

Three. Experiment Method

Four. Samples Delivery Requirements

Sample Type	Sample Requirements	Delivery conditions
Tissue	The isolated tissues are made into cell suspension and added into PBS medium	At ambient temperature
Living cell	Sample amount: 1*10^6 cells/TEST, add into PBS medium	At ambient temperature
Blood	Collect the blood in the anticoagulant blood collection vessel of EDTA heparin sodium.	At ambient temperature

Five. Case Display

Six. Common Problems

1. Preparation method of BrdU: dissolve 10mg in 10ml double distilled water and store it away from light at 4 ℃.

2. BrdU will cause irreversible damage to the body. Be cautious during use and avoid inhaling BrdU dust.

Seven. Service Process

Primary Cell Preparation and Culture

Transwell Migration/Invasion Assays