Fresh tissue

The tissue is collected newly and freshly; after the blood is cleared away with PBS, the tissue is preserved at -80°C immediately

At -80°C

With dry ice

All samples need to be uniquely marked and the markings are identifiable

Regular fixed tissue

The volume of the tissue is within 2cm*2cm*0.5cm in general. The tissue is fixed to keep its original shape as much as possible and clean up the blood and excess parts; the volume of fixative fluid should be at least 10 times the tissue, and the container should be big enough to hold the tissue without squeezing it. Label the fixative fluid type and the fixed duration.

At ambient temperature

At ambient temperature

Special fixed tissue

For tissue of testicle, eyeball, spinal cord, muscle, and so on: the correspondent special fixative fluid is recommended for fixation to ensure the fixing effect.

Paraffin-

embedded tissue

The tissue should be embedded with a standard embedding cassette and evenly combined without crack; the thickness of the wax block depends on the number of slices and the effective thickness should be over 0.1 cm. Provide the paraffin-embedded tissue made within six months. Otherwise, the antigens of the paraffin may be lost and fail to detect the protein when performing immunohistochemistry.

At ambient temperature

At ambient temperature

Tissue slices

It needs to pick up the section with anti-drop slides and label the thickness of the slice, the temperature, and the baking duration. The tissue should be near the lower third of the slice.

At 4°C

With ice bag

Cell climbing slices

Use cell climbing film for the well plate. Wash the cell climbing film with PBS 2 to 3 times after fixation, store it in PBS, and seal the well plate with sealing film. Each well plate cover of the film should be marked uniquely, and electronic grouping information is required to prevent the mark from being blurred during transportation.

At ambient temperature

At ambient temperature

Plant example

Fix the fresh tissue with FAA and its degree of lignification should not be high; for thinner blades, if parallel blade slicing is required, it is difficult to ensure its integrity; the diameter of the rhizome should be larger than 1 mm.  

At ambient temperature

At ambient temperature

Antibody

A clear mark to identify the antigen is required on the containers and the instructions should be attached. The amount of antigens should be more than that of the experiment required.

For tissue slices and tissue chips, calculate the amount of antibody according to 100 μl/slice after dilution; for the round coverslips of the 24 well plate cell climbing slide, calculate the amount of antibody according to 200 μl/slice after dilution; for the round coverslips of the 12 well plate cell climbing slide, calculate the amount of antibody according to 400 μl/slice after diluted

At -20°C

With ice bag or dry ice